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Shields, M.S., S.O. Montgomery, P.J. Chapman, S.M. Cuskey and P.H. Pritchard. 1988. Trichloroethylene Degradation via a Novel Pathway of Toluene Catabolism by Strain G4. In: Reducing Uncertainty in Environmental Risk Assessment: Abstracts: 9th Annual Meeting, 13-17 November 1988, Arlington, VA. Society of Environmental Toxicology and Chemistry, Washington, DC. Pp. 152. (ERL,GB R082).

The cometabolic capacity of gram negative aerobic bacteria to degrade trichloroethylene (TCE) when induced with toluene or phenol has been investigated. The ability of Pseudomonas putida F1 to accomplish this via toluene dioxygenase has been established. We report here a novel toluene catabolic pathway of the environmental isolate strain G4 that allows the co-metabolic mineralization of TCE. HPLC and GC-MS analyses of culture supernatants of G4 wild type and mutants established the presence of both o- cresol (OC) and 3-methylcatechol (3MC) as products of toluene catabolism. Transient formation of OC preceded accumulation of 3MC by a mutant blocked in 3MC catabolism. In addition toluene cis-dihydrodiol was never detected. This clearly defined the reaction sequence of toluene oxidation. A single mutant class unable to catabolize toluene, or OC was also unable to degrade TCE. Spontaneous revertants of this class regained all catabolic and TCE degradative capabilities. Strain G4 appears to degrade TCE via a monooxygenase involved in the formation of OC from toluene.

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