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Research Product

Mulbry, Walter W. and Richard W. Eaton. 1991. Purification and Characterization of the N-Methylcarbamate Hydrolase from Pseudomonas Strain CRL-OK. Appl. Environ. Microbiol. 57(12):3679-3682. (ERL,GB X740).

A unique cytosolic enzyme that hydrolyzes the carbamate linkage of the insecticide carbaryl (1-naphthyl N-methylcarbamate) was purified from extracts of Pseudomonas sp. strain CRL-OK. Substrates of the hydrolase include the N-methylcarbamate pesticides carbofuran and aldicarb but not the phenylcarbamate isopropyl m-chlorocarbanilate, the thiocarbamate S-ethyl N, N-dipropylthiocarbamate, or the dimethylcarbamate o-nitrophenyldimethylcarbamate.

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