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BIOSCREEN is a screening model that simulates remediation through natural attenuation of dissolved hydrocarbons at petroleum fuel release sites. The model is designed to simulate biodegradation by both aerobic and anaerobic reactions.

This software, programmed in the Microsoft Excel spreadsheet environment and based on the Domenico analytical solute transport model, has the ability to simulate advection, dispersion, adsorption, and aerobic decay, as well as anaerobic reactions that have been shown to be the dominant biodegradation processes at many petroleum release sites. BIOSCREEN includes three different model types:


Version 1.4
Release Date July 1997
Development Status general release
Development Information  
Operating System Windows 95/98/NT, Excel
Development Language  
Intended Audience  
Key Words  
Related Web Sites  

Minimum System Requirements

Any computer system capable of running Microsoft Excel version 5.0 or later will be able to run BIOSCREEN. However, because of the intensive floating point calculations used in the model, GSI recommends that the following minimum requirements be observed to ensure a minimum standard of performance:

Hardware Requirements

Software Requirements


Installation program, help files, and instructions (ZIP)(1.3 MB)

US EPA. (1996) "BIOSCREEN, Natural Attenuation Decision Support System – User’s Manual, Version 1.3." Publication No. EPA/600/R-96/087. August 1996

  1. Refer to the user's manual for further instructions.
  2. Go to the Windows Explorer and create a directory on the C drive named BIOSCRN (C:\BIOSCRN).
  3. Download the user’s manual and installation zip file to the new BIOSCRN directory.
  4. Extract the contents of the installation zip file inside the C:\BIOSCRN directory.
  5. To run BIOSCREEN after installation, start Microsoft Excel and open the BIOSCRN4.xls file. Loading from within Excel is preferred to double-clicking the file name in the file manager.

You will need Adobe Reader to view some of the files on this page. See EPA's PDF page to learn more.

Office of Research & Development | National Risk Management Research Laboratory

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