Research Product

The bioassay procedures given in this manual were developed to provide tests for conducting toxicity evaluations of waste materials considered for ocean disposal under EPA's Ocean Disposal Permit Program. Nine bioassay procedures are described; three of which are considered 'special' and are not recommended for routine use. The procedures specify the use of various organisms representing several trophic levels. Both flow-through and static tests are included. Methods given vary in their utility and complexity of performance. The procedures are not intended to be considered 'standard methods', but are to be used as reference methods or official methods dependent on the judgement of the EPA Regional Administrator responsible for the management of the permit program.