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Microbiological and Chemical Exposure Assessment

Cryptosporidium Image Sequence 1

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Differential interference contrast (DIC) image of Cryptosporidium parvum

Photo Credit: H.D.A Lindquist, U.S. EPA

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Differential interference contrast (DIC) image of Cryptosporidium parvum oocysts, purified from murine fecal material. Oocysts are spheroidal objects, 4 to 6 microns in diameter. Oocysts may contain as many as 4 banana shaped sporozoites, and a residual body. In some oocysts the sporozoites and residuum will be indistinct. Scale bar is 10 microns.

Immunofluorescence image of Cryptosporidium parvum

Photo Credit: H.D.A Lindquist, U.S. EPA

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Immunofluorescence image of Cryptosporidium parvum oocysts, purified from murine fecal material. (Same field of view) Oocysts were stained with commercially available immunofluorescent antibodies. Oocysts should have an intense apple green fluorescence on the periphery of their oocyst wall, and measure 4 to 6 microns in diameter. Scale bar is 10 microns.

Fluorescence image of Cryptosporidium parvum

Photo Credit: H.D.A Lindquist, U.S. EPA

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Fluorescence image of Cryptosporidium parvum oocysts, purified from murine fecal material. (Same field of view.) Oocysts were stained with 4,6-diamidino 2-phenyl-indole dihydrochloride (DAPI). DAPI interacts with nucleic acids and stains the nucleus of each sporozoite within the oocyst. There should be 4 sporozoites each with 1 nucleus, or 4 stained nuclei in each oocyst. Oocysts that appear to have fewer than 4 stained nuclei, may have  4 nuclei with the others not visible in this plane of focus. Oocysts with no nuclei visible, may be dead, may be resistant  to DAPI staining, or may be organisms other than C. parvum.

Photo Credit: These images are in the public domain and are thus free of any copyright restrictions. For public domain images, permission is not required for their use but we do ask that you credit the EPA and the contributors (found in the Photo Credit line) whenever an image is used in any publicly distributed media.

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