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Microbiological and Chemical Exposure Assessment Staff


Dawn King

Title: Microbiologist

EPA Division: Microbiological and Chemical Exposure Assessment Research Division

EPA Branch: Microbial Exposure Research Branch

Narrative:
Developed immunological techniques to detect target organisms and toxins by immunoassay and flow cytometry using drinking water, recreational water, and various other matrices. Development and optimization of methods to detect microbial contaminants in water by real-time PCR. Optimization of the U.S.EPA Method 1623: Cryptosporidium and Giardia in water by Filtration/IMS/FA. Conducting training to assist commercial laboratories to utilize Method 1623.

Education/Training:

  • B.S. Microbiology, Miami University, 2002

Professional Experience:

  • Microbiologist, USEPA, ORD, NERL, MCEARD, MERB, Cincinnati, OH 2002-Present
  • Research Microbiologist, USEPA, OW, TSC, Cincinnati, OH 2002-2002

Select Presentations:

  • King, D., Donohue, M., Pfaller, S. 2010. Detection of Mycobacterium avium and Mycobacterium intracellulare Nationwide from Drinking Water Taps Using Quantitative Real-Time PCR. U.S. EPA. American Society for Microbiology, 110th General Meeting, San Diego, CA.
  • King, D., Beumer, A., Pfaller, S. 2007. Real-Time quantitative PCR detection of mycobacterium avium complex organisms in drinking water, poster presentation at the General Meeting of the American Society for Microbiology, Toronto, Canada.
  • King, D., Beumer, A., Pfaller, S. 2007. Identification of Mycobacterium avium subsp. hominissuis Isolated from Drinking Water and Food. U.S. EPA. American Water Works Association, Water Quality Technology Conference, Charlotte, North Carolina.
  • King, D., Beumer, A., Pfaller, S. 2007. Real-Time Quantitative PCR Detection of Mycobacterium avium Complex Organisms in Drinking Water. U.S. EPA. American Society for Microbiology, 107th General Meeting, Toronto, Canada.
  • Pfaller, S.L., D. King, and T.C. Covert, 2006. Detection and Quantification of Mycobacterium Avium Complex Organisms in Water. U.S. EPA. MCEARD Peer Review.
  • Stang, D., K. Brenner, and M. Rodgers, 2005. A Critical Evaluation of Flow Cytometry Used for Detecting Enterococcus faecalis and Enterococcus faecium in Recreational Waters. U.S. EPA. American Society for Microbiology, 105th General Meeting, Atlanta, Georgia.
  • Stang, D., C. Davis, R. Findlay, J. Flood, P. Ganz, and G. Ridder. 2002. Development of a Rapid Immunoassay to Measure Toxic Shock Syndrome Toxin-1 in Two Different Matrices. The Procter and Gamble Company and Miami University. American Society for Microbiology, 102nd General Meeting, Salt Lake City, UT.

Published & Presented Research (1999-Present)

Contact Info:

Dawn King
Microbiological and Chemical Exposure Assessment Research Division
National Exposure Research Laboratory
Office of Research and Development
U.S. Environmental Protection Agency
27 W. Martin Luther King Jr. Drive
Cincinnati, OH 45268
Telephone: 513-569-7093
Email: king.dawn@epa.gov

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