Microbiological and Chemical Exposure Assessment Staff
EPA Division: Microbiological and Chemical Exposure Assessment Research Division
EPA Branch: Microbial Exposure Research Branch
My research is aimed at developing a fast rapid method, qPCR, for the detection of enterococci indicator bacteria. Currently, cultural methods are utilized and require 24 hours before results are read. QPCR provides results in a few hours so the same day a sample is taken results can be analyzed. No longer will today’s results be for yesterday’s sample.
- B.S. Biology, Heidelberg College, 1991.
- Biologist, USEPA, ORD, NERL-MCEARD, Cincinnati, OH 1998-present
- Biological Lab Technician, USEPA, Office of Pesticide Programs, Cincinnati, OH 1995-1998
Select Awards and Honors:
- S-Award (2011), Award for Contribution to Recreational Waters Research
- Scientific and Technological Achievement Award, Level 1 (STAA) (2010)-Development of a Molecular biology Data Analysis Model
- Scientific and Technological Achievement Award, Level 1 (STAA) (2010)-The Development of Quantitative Real-Time PCR Methods for the Detection of Human and Cow Fecal Pollution
- Scientific and Technological Achievement Award, Level 1 (STAA) (2006)-Protecting Swimmers’ Health with Faster Ways of Measuring Water Quality
- Quality Step Increase (2005), Award for High Quality Performance
- S-Award (2004), Award for Participating in Rapid Methods Competition
- S-Award (2003), Group Award with Richard Haugland for assisting in the Beach Progam’s Epi-Study.
- Team Award (2001), for Rapid Molecular Method Development pertaining to the Bathing Beach Program.
- Outstanding Performance Appraisal and Monetary Award (1996,1997).
- Evaluation of the sensitivity and specificity of Enterococcus qPCR, poster presentation at Enterococci Meeting of the American Society for Microbiology, 2010, Portland, OR. Luis A.Ríos-Hernández, S. Siefring, E.C. Chern, and R.A. Haugland.
- Effects of long-term freezer storage on quantitative PCR-measured Enterococcus DNA sequence levels in surface water filter retentates, 2008, Great Lakes Beach Conference, Porter, IN. S. Siefring, L. Wymer and R. Haugland.
- Internal Amplification Control for use in Quantitative Polymerase Chain Reaction Fecal Indicator Bacteria Assays, poster presentation at the Regional Meeting of the American Society for Microbiology, 2007, Canton, Ohio. S.Siefring, E. Atikovic, R.A.Haugland, M. Sivaganesan, and O.C.Shanks.
- New Target and Control Assays for Quantitative Polymerase Chain Reaction (QPCR) Analysis of Enterococci in Water, 2005, Atlanta, Georgia. SD Siefring, LJ Wymer, and RA Haugland.
- Detection of Fecal Enterococci Using A Real Time PCR Method, poster presentation at the General Meeting of the American Society for Microbiology, 2001, Orlando, Florida. SD Siefring, JW Santo Domingo, N Brinkman, and RA Haugland.
Microbiological and Chemical Exposure Assessment Research Division
National Exposure Research Laboratory
Office of Research and Development
U.S. Environmental Protection Agency
27 W. Martin Luther King Jr. Drive
Cincinnati, OH 45268