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OCSPP Harmonized Test Guidelines

Series 835 - Fate, Transport and Transformation Test Guidelines

The FINAL guidelines on this page are part of a series of test guidelines that have been developed by the Office of Chemical Safety and Pollution Prevention (OCSPP) for use in the testing of pesticides and toxic substances, and the development of test data for submission to the Agency. 

A Master List (PDF) (28 pp, 80K, About PDF) of the OCSPP Harmonized Test Guidelines is available | Microsoft Excel Version (84K) (Excel viewer Exit EPA Disclaimer)

More information about OCSPP Harmonized Test Guidelines.

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Group A — Laboratory Transport Test Guidelines

835.0001 - Principles and Strategies Related to Biodegradation Testing of Organic Chemicals under the Toxic Substances Control Act (TSCA) (November 2008)
835.1110 - Activated Sludge Sorption Isotherm (January 1998)
835.1210 - Soil Thin Layer Chromatography (January 1998)
835.1220 - Sediment and Soil Adsorption/Desorption Isotherm (January 1998)
835.1230 - Adsorption/Desorption (Batch Equilibrium) (November 2008)
835.1240 - Leaching Studies (November 2008)
835.1410 - Laboratory Volatility (November 2008)

Group B — Laboratory Abiotic Transformation Test Guidelines

835.2120 - Hydrolysis (November 2008)
835.2130 - Hydrolysis as a Function of pH and Temperature (January 1998)
835.2210 - Direct Photolysis Rate in Water by Sunlight (January 1998))
835.2240 - Photodegradation in Water (November 2008)
835.2310 - Maximum Direct Photolysis Rate in Air from UV/Visible Spectroscopy (January 1998)
835.2370 - Photodegradation in Air (November 2008)
835.2410 - Photodegradation in Soil (November 2008)

Group C — Laboratory Biological Transformation Test Guidelines

835.3100 - Aerobic Aquatic Biodegradation (January 1998)
835.3110 - Ready Biodegradability (January 1998)
835.3140 - Ready Biodegradability – CO2 in Sealed Vessels (Headspace Test) (November 2008) [SUPERSEDES 3120]
835.3160 - Biodegradability in Sea Water (January 1998)
835.3170 - Shake Flask Die-Away Test (January 1998)
835.3180 - Sediment/Water Microcosm Biogradation Test (January 1998)
835.3190 - Aerobic Mineralization in Surface Water – Simulation Biodegradation Test (October 2008)
835.3200 - Zahn-Wellens/EMPA Test (January 1998)
835.3210 - Modified SCAS Test (January 1998)
835.3215 - Inherent Biodegradability – Concawe Test (October 2008)
835.3220 - Porous Pot Test (January 1998)
835.3240 - Simulation Test – Aerobic Sewage Treatment: A. Activated Sludge Units (October 2008)
835.3260 - Simulation Test – Aerobic Sewage Treatment: B. Biofilms (October 2008)
835.3280 – Simulation Tests to  Assess the Primary and Ultimate Biodegradability of Chemicals Discharged to Wastewater (October 2008)
835.3300 - Soil Biodegradation (January 1998)
835.3400 - Anaerobic Biodegradability of Organic Chemicals (January 1998)
835.3420 - Anaerobic Biodegradability of Organic Compounds in Digested Sludge: By Measurement of Gas Production (October 2008)

Group D —Transformation in Water and Soil Test Guidelines

835.4100 - Aerobic Soil Metabolism / 835.4200 – Anaerobic Soil Metabolism (October 2008)
835.4300 - Aerobic Aquatic Metabolism / 835.4400 – Anaerobic Aquatic Metabolism (October 2008)

Group E — Transformation Chemical-Specific Test Guidelines

835.5045 - Modified SCAS Test for Insoluble and Volatile Chemicals (January 1998)
835.5154 - Anaerobic Biodegradation in the Subsurface (January 1998)
835.5270 - Indirect Photolysis Screening Test: Sunlight Photolysis in Waters Containing Dissolved Humic Substances (January 1998)

Group F — Field Dissipation Test Guidelines

835.6100 - Terrestrial Field Dissipation (October 2008)
835.6200 - Aquatic (Sediment) Field Dissipation (October 2008)
835.6300 - Forestry Dissipation (October 2008)
835.6400 - Combination and Tank Mixes Field Dissipation (October 2008)

Group G — Ground Water Monitoring Test Guidelines

835.7100 - Guidance for Prospective Ground-Water Monitoring Studies

Group H — Volatility from Soil Test Guidelines

835.8100 - Field Volatility (November 2008)

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