Jump to main content.


Analytical Methods Developed by the Office of Ground Water and Drinking Water

You will need Adobe Reader to view some of the files on this page. See EPA's PDF page to learn more.

The Office of Ground Water and Drinking Water's Technical Support Center (TSC) is one of the EPA offices responsible for developing analytical methods for drinking water. TSC developed, or participated in the development of, many methods for determining the concentrations of chemical and microbial contaminants in drinking water.

Many of the chemical methods developed by TSC are approved for analyses of compliance or unregulated contaminant monitoring samples under the Safe Drinking Water Act. Laboratories can verify that a method is approved by checking the Code of Federal Regulations (40 CFR 141). Listings of approved methods for each regulation are also available on the Analytical Methods Web page. Questions or comments about these methods should be addressed to the Safe Drinking Water Hotline at 1-800-426-4791, or online.


Individual Chemical Methods Available for Download

EPA Method Number Method Title
300.11 Determination of Inorganic Anions in Drinking Water by Ion Chromatography PDF (40pp, 408K)
302.0 Determination of Bromate in Drinking Water Using Two-Dimensional Ion Chromatography with Suppressed Conductivity Detection PDF (38 pp, 509K)
314.01 Determination of Perchlorate in Drinking Water by Ion Chromatography PDF (49pp, 270K)
314.1 Rev 1.0 Determination of Perchlorate in Drinking Water Using Inline Column Concentration/Matrix Elimination Ion Chromatography with Suppressed Conductivity Detection PDF (38pp, 568K)
314.2 Determination of Perchlorate in Drinking Water Using Two- Dimensional Ion Chromatography with Suppressed Conductivity Detection PDF (42 pp, 667K)
317.0 Rev. 2.0 Determination of Inorganic Oxyhalide Disinfection By-products in Drinking Water Using Ion Chromatography with the Addition of a Postcolumn Reagent for Trace Bromate Analysis PDF (49 pp, 14MB)
326.0 Determination of Inorganic Oxyhalide Disinfection By-Products in Drinking Water Using Ion Chromatography Incorporating the Addition of a Suppressor Acidified Postcolumn Reagent for Trace Bromate Analysis PDF (47 pp, 3.3MB)
327.0 Rev. 1.1 Determination of Chlorine Dioxide and Chlorite Ion in Drinking Water Using Lissamine Green B and Horseradish Peroxidase with Detection by Visible Spectrophotometry PDF (30 pp, 191K) (Typographical errors in Revision 1.0 are corrected in Sections 7.2.4.2, 7.2.5, and 7.3.1.)
331.0 Rev. 1.0 Determination of Perchlorate in Drinking Water by Liquid Chromatography Electrospray Ionization Mass Spectrometry PDF (34 pp, 197K)
334.0 Determination of Residual Chlorine in Drinking Water Using an On-line Chlorine Analyzer PDF (24 pp, 174K)
515.31 Determination of Chlorinated Acids in Drinking Water by Liquid-Liquid Extraction, Derivatization and Gas Chromatography with Electron Capture Detection PDF (56 pp, 132K)
515.4 Determination of Chlorinated Acids in Drinking Water by Liquid-Liquid Microextraction, Derivatization, and Fast Gas Chromatography with Electron Capture Detection PDF (48 pp, 4.5MB)
524.3 Measurement of Purgeable Organic Compounds in Water by Capillary Column Gas Chromatography/Mass Spectrometry PDF (55 pp, 864K)
5261 Determination of Selected Semivolatile Organic Compounds in Drinking Water by Solid Phase Extraction and Capillary Column Gas Chromatography / Mass Spectrometry (GC/MS) PDF (49 pp, 169K)
527 Rev. 1.0 Determination of Selected Pesticides and Flame Retardants in Drinking Water by Solid Phase Extraction and Capillary Column Gas Chromatography / Mass Spectrometry (GS/MS) PDF (43 pp, 326K) - (Minor Corrections, August 2009)
531.2 Measurement of N-Methylcarbamoyloximes and N-Methylcarbamates in Water by Direct Aqueous Injection HPLC with Postcolumn Derivatization PDF (38 pp, 1.2MB)
5321 Determination of Phenylurea Compounds in Drinking Water by Solid Phase Extraction and High Performance Liquid Chromatography with UV Detection PDF (47 pp, 107K)
536 Determination of Triazine Pesticides and Their Degradates in Drinking Water by Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry (LC/ESI-MS/MS) PDF (32 pp, 299K)
552.3 Determination of Haloacetic Acids and Dalapon in Drinking Water by Liquid-Liquid Microextraction, Derivatization, and Gas Chromatography with Electron Capture Detection PDF (55 pp, 636K)
5561 Determination of Carbonyl Compounds in Drinking Water by Pentafluorobenzylhydroxylamine Derivatization and Capillary Gas Chromatography with Electron Capture Detection PDF (38 pp, 140K) (This method was jointly developed with EPA’s Office of Research and Development.)
556.11 Determination of Carbonyl Compounds in Drinking Water by Fast Gas Chromatography PDF (37 pp, 185K)
557 Determination of Haloacetic Acids, Bromate, and Dalapon in Drinking Water by Ion Chromatography Electrospray Ionization Tandem Mass Spectrometry (IC-ESI-MS/MS) PDF (43 pp, 1.1M)

1 This method is published in "Methods for the Determination of Organic and Inorganic Compounds in Drinking Water, Volume 1" (EPA 815-R-00-014) which is available through EPA’s digital publications National Environmental Publications Internet Site (NEPIS) database.


Top of page

Additional Chemical Methods

Additional Chemical Methods listed below are included inMethods for the Determination of Organic Chemicals in Drinking Water, Supplement III" (EPA/600/R-95/131) which is available through EPA’s digital publications National Environmental Publications Internet Site (NEPIS) database.

Most of the individual methods are also available for download from the National Environmental Methods Index (NEMI).

EPA Method Number Method Title
504.1 1,2-Dibromoethane (EDB), 1,2-Dibromo-3-Chloropropane (DBCP), and 1,2,3-Trichloropropane (123TCP) in Water by Microextraction and Gas Chromatography  (Available for download from NEMI)
507 Determination of Nitrogen- and Phosphorus-Containing Pesticides in Water by Gas Chromatography with a Nitrogen-Phosphorus Detector (Available for download from NEMI)
508 Determination of Chlorinated Pesticides in Water by Gas Chromatography with an Electron Capture Detector (Available for download from NEMI)
508.1 Determination of Chlorinated Pesticides, Herbicides, and Organohalides by Liquid-Solid Extraction and Electron Capture Gas Chromatography (Available for download from NEMI)
509 Determination of Ethylene Thiourea (ETU) in Water using Gas Chromatography with a Nitrogen-Phosphorus Detector
515.1 Determination of Chlorinated Acids in Water by Gas Chromatography with an Electron Capture Detector
531.1 Measurement of N-Methylcarbamoyloximes and N-Methylcarbamates in Water by Direct Aqueous Injection HPLC with Post Column Derivatization (Available for download from NEMI)
551.1 Determination of chlorination disinfection Byproducts, Chlorinated Solvents, and Halogenated Pesticides/Herbicides in Drinking Water by Liquid-Liquid Extraction and Gas Chromatography with Electron-Capture Detection (Available for download from NEMI)
552.2 Determination of Haloacetic Acids and Dalapon in Drinking Water by Liquid-Liquid Extraction, Derivatization and Gas Chromatography with Electron Capture Detection (Available for download from NEMI)

Top of page

Individual Microbial Methods Available for Download

EPA Method Number Method Title
1605 Aeromonas in Finished Water by Membrane Filtration using Ampicillin-Dextrin Agar with Vancomycin (ADA-V) PDF (36pp, 443K) (This method was jointly developed with EPA’s Office of Research and Development.)

Top of page

Lowest Concentration Minimum Reporting Level (LCMRL)

For the past several years, EPA has been working on a procedure to determine the Lowest Concentration Minimum Reporting Level (LCMRL). The LCMRL is defined as the lowest spiking concentration such that the probability of spike recovery in the 50% to 150% range is at least 99%. LCMRLs are laboratory- and method-specific, and were used by EPA to determine Minimum Reporting Levels (MRLs) for use in the second Unregulated Contaminant Monitoring Regulation (UCMR 2). In addition, EPA’s Technical Support Center (TSC) personnel are looking into the potential to expand the use of the LCMRL in EPA programs. Laboratories will only be required to demonstrate their ability to quantitate at the MRLs specified in 40 CFR 141.40. Therefore, there is no requirement, nor associated financial burden, for laboratories to determine LCMRLs.

EPA recently revised the equations within the LCMRL calculator that perform the data regression. Rather than using linear or weighted linear regressions, the revised LCMRL calculator uses advanced statistics to minimize the error at each concentration. The revised calculator is currently undergoing peer review and will be posted to this site when that review is completed. A report describing LCMRL and the current LCMRL calculator are available electronically below.


Top of page

Workshops on Microbial Methods Development

Workshop on the Large Volume Sample Preparation for Waterborne Pathogens, April 4-5, 2006

Workshop on the Feasibility of Using DNA/RNA Microarrays and Related Technologies for High Through-Put Detection of Waterborne Pathogens, March 22-23, 2005

Workshop to Develop a Protocol for Reliable Genetic Methods for the Detection of Viruses, for Use in EPA’s Water Programs, January 15-16, 2003

Top of page

Safewater Home | About Our Office | Publications | Questions and Answers | Links | Office of Water | En Español


Local Navigation


Jump to main content.