Pacific Southwest, Region 9
Serving: Arizona, California, Hawaii, Nevada, Pacific Islands, Tribal Nations
Laboratory Standard Operating Procedures
Note: To obtain a copy of a specific SOP please contact:
Lucrina Jones (Jones.Lucrina@epa.gov)
(510) 412-2321
| SOP# | SOP TITLE | |
|---|---|---|
| ADMINISTRATIVE: PURCHASING, SHIPPING/RECEVING & LAB CERTIFICATION | ||
| 010 | Guidance for use of EPA Purchase Cards | |
| 020 | Shipping and Receiving of Coolers, Supplies and Equipment | |
| 030 | Sample Coordination by the RSCC | |
| 050 | State Drinking Water Laboratory Certification Program Oversight | |
| SAMPLE CONTROL | ||
| 110 | Sample Receipt, Login, and Storage | |
| 112 | Sample Receiving in the Absence of the Sample Custodian | |
| 115 | Chemical Preservation upon Receipt at the Laboratory | |
| 125 | Aqueous Sample Disposal | |
| 130 | Glassware Cleaning Procedures | |
| 150 | Soil and Sediment Homoginization | |
| CHEMISTRY: ORGANIC SAMPLE AND STANDARDS PREPARATION | ||
| 205 | Preparation of Organic-free Method Blank Water | |
| 239 | Recycling Used Methylene Chloride | |
| 260 | Gel-Permeation Chromatography (GPC) Clean-up | |
| 275 | Extraction of Water Samples using CLLE | |
| 285 | Soxhlet Extraction of Solid Samples | |
| 290 | Extraction of Soil Samples Using PFE (Pressurized Fluid Extraction - ASE) | |
| 295 | Waste Dilution | |
| CHEMISTRY: ORGANIC SAMPLE ANALYSIS | ||
| 305 | Volatile Organics Analysis | |
| 311 | Analysis of Volatile Organic Compounds in Air and Soil Vapor | |
| 312 | SUMMA Canister Leak Check and Cleaning | |
| 314 | TO15 SIM-Analysis of Volatile Organic Compounds in Air | |
| 315 | Semivolatile Organics Analysis | |
| 325 | Determination of Dissolved Gases in Water | |
| 330 | Pesticides & PCB (Water, Sediment, Soil) by GC | |
| 335 | Polychlorinated Biphenyls (PCBs) by GC | |
| 350 | Analysis of EDB and DBCP in Water by Micro-extraction and GC | |
| 353 | Low-level Volatiles | |
| 354 | Volatile Organic Analysis in Water | |
| 375 | Low-level Semi-Volatile Organics Analysis | |
| 380 | Purgeable Aromatics and Hydrocarbons by GC PID/FID | |
| 385 | TPH/DRO Analysis by GC/FID | |
| CHEMISTRY: INORGANIC SAMPLE AND STANDARDS PREPARATION | ||
| 250 | TCLP Extraction | |
| 252 | California Waste Extraction Test (WET) | |
| 254 | SPLP Extraction | |
| 256 | Bioaccessibility Extraction | |
| 401 | Nepholometric Turbidity Measurement | |
| 403 | Aqueous Sample Preparation for ICP-AES and ICP-MS | |
| 405 | Preparation of Solid Samples for Metals Analysis | |
| 407 | Preparation of Leachate | |
| 409 | Digestion for Lead on Filters | |
| 420 | Microwave Digestion of Solid Samples | |
| 430 | Tissue Preparation and Homogenization | |
| CHEMISTRY: INORGANIC SAMPLE ANALYSIS | ||
| 460 | Percent Solids Determination | |
| 461 | Total Dissolved Solids | |
| 462 | Total Suspended Solids | |
| 463 | Settleable Solids | |
| 464 | Suspended Sediment Concentration | |
| 475 | Flash Point by Pensky-Martens Closed Tester | |
| 503 | Analysis of Solids by ICP | |
| 505 | Determination of Trace Metals in Water by ICP-AES | |
| 507 | Trace Metals by ICP-MS | |
| 509 | Determination of Lead on Filters by ICP-MS | |
| 515 | Mercury Analysis by Cold Vapor Atomic Absorption | |
| 517 | Analysis of Solid Samples for Mercury | |
| 525 | Hexavalent Chromium in Water by Ion Chromatography | |
| 530 | Anions by Ion Chromatography | |
| 531 | Analysis of Perchlorate by Ion Chromatography | |
| 532 | Cyanide Analysis | |
| 535 | Mercury in Solids by Thermal Decomposition, Amalgamation and AAS | |
| 537 | Analysis of Perchlorate by LC-MS-MS | |
| 545 | Specific Conductance | |
| 553 | Determination of TOC in Water by EPA Method 415.3 | |
| 560 | Total Alkalinity by Titrimetric Method | |
| 565 | Sulfide Analysis by Titration | |
| 570 | Hardness, Total (mg/l as CaCO3) | |
| 575 | Determination of Ferrous Iron by Titration with Dichromate | |
| 580 | pH Determination | |
| 582 | pH in Solid Samples | |
| 590 | Ammonia Analysis | |
| 592 | Total Kjeldahl Nitrogen in Water | |
| 594 | Analysis of Nitrite and Nitrate in Water by Cadmium Reduction | |
| 596 | Analysis of Total Phosphorus in Water | |
| SAFETY, HEALTH & ENVIRONMENTAL COMPLIANCE: STANDARD PRACTICES | ||
| 704 | Inspection Procedures for Emergency Eyewash and Shower Units | |
| 706 | Laboratory Waste Management | |
| 712 | Inspection Procedures for the Satellite and 90-Day Hazardous Waste Accumulation Areas | |
| 716 | Cleaning and Recycling of Empty Containers | |
| 730 | Neutralization of Aqueous Wastes | |
| 745 | Maintenance and Inspection of Spill Cleanup Equipment | |
| 747 | Laboratory Chemical Inventory Maintenance | |
| 748 | Chemical Adoption Program | |
| 750 | Inspection Procedures for Fire Extinguishers | |
| 760 | Maintenance and Upkeep of Radioactive Sources | |
| 765 | Procedure for Spill Response and Exposure Control for Methylene Chloride | |
| 770 | Handling Cryogenic Materials | |
| 775 | Handling and Disposal of Broken Glassware | |
| 780 | Procedure for Chemical Spill Response and Cleanup | |
| 785 | Procedure for Storage, Monitoring and Handling of Peroxide Forming Chemicals | |
| QUALITY ASSURANCE | ||
| 805 | Refrigerator temperature monitoring | |
| 810 | Internal Audits of Laboratories | |
| 817 | Accuracy Check of Laboratory Thermometers | |
| 820 | Lab Discrepancy/Corrective Action Report Procedure | |
| 825 | Deionized water monitoring | |
| 830 | Notification of exceeding safe drinking water mcl | |
| 835 | Chromatographic integration procedures | |
| 840 | Notebook documentation and control | |
| 845 | Final Chemistry Data Review & Report Preparation | |
| 850 | Preparation and usage of SOPs | |
| 880 | Initial & continuing demonstrations of proficiency (Chemistry) | |
| 885 | Initial & continuing demonstrations of proficiency (Biology) | |
| 890 | Calibration & maintenance of automatic pipets | |
| 892 | Initial Instrument Calibration Guidelines | |
| BIOLOGY: MARINE TECHNICAL | ||
| 1001 | Sea urchin & sand dollar fertilization toxicity test | |
| 1004 | Red abalone larval development toxicity test | |
| BIOLOGY: FRESHWATER TECHNICAL - CHRONIC | ||
| 1020 | Fathead minnow, larval survival & growth test | |
| 1022 | Green alga, growth test | |
| BIOLOGY: FRESHWATER TECHNICAL - ACUTE | ||
| 1030 | Fathead minnow acute survival test | |
| 1032 | Daphnia magna acute survival test | |
| BIOLOGY: GENERAL ACTIVITY | ||
| 1060 | Calibration and Operation of pH meter | |
| 1061 | Calibration and Operation of Dissolved Oxygen Meters | |
| 1063 | Calibration and Operation of Conductivity Meters | |
| 1067 | Calibration and Operation of Refractometers | |
| 1073 | Calibration & Operation of Oakton Portable pH/CON 10 Series Meter | |
| 1080 | Preparation of standard dilution water (freshwater) | |
| 1081 | Preparation of foods (freshwater) | |
| 1082 | Culturing of selenastrum capricornatum | |
| 1085 | Glass & Plasticware Cleaning for Marine Toxicity Testing | |
| 1086 | Kelp collection, preparation & feeding | |
| BIOLOGY: MICROBIOLOGY | ||
| 1101 | Membrane filtration coliform analysis | |
| 1102 | Multiple-tube fermentation analysis for total and fecal coliform | |
| 1103 | Colilert coliform and E. coli water analysis | |
| 1106 | Volunteer monitoring of surface waters for bacteria | |
| 1107 | Enterolert entercocci water analysis | |
| 1108 | Heterotrophic plate count for bacteria in water | |
| 1109 | Microbiology Autoclaves | |
| BIOLOGY: MISCELLANEOUS BIOLOGY | ||
| 1131 | Analysis of Ash Free Dry Mass | |
| 1133 | Biochemical oxygen demand-BOD | |
| 1134 | Chlorophyll | |
| 1135 | Total & free residual chlorine - Hach DPD | |
| FIELD SAMPLING: WATER | ||
| 1220 | Groundwater Monitoring Well Sampling | |
| 1222 | Routine Sampling and Analysis of Drinking water | |
| MISCELLANEOUS: IMMUNOASSAY | ||
| 1305 | Microcystin by ELISA Analysis | |
| Pacific Southwest NewsroomPacific Southwest Programs | Grants & FundingUS-Mexico Border | Media Center Careers | About EPA Region 9 (Pacific Southwest)A-Z Index |