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Pacific Southwest, Region 9

Serving: Arizona, California, Hawaii, Nevada, Pacific Islands, Tribal Nations

Laboratory Standard Operating Procedures

Note: To obtain a copy of a specific SOP please contact:

Lucrina Jones (Jones.Lucrina@epa.gov)
(510) 412-2321
SOP# SOP TITLE
ADMINISTRATIVE: PURCHASING, SHIPPING/RECEVING & LAB CERTIFICATION
010 Guidance for use of EPA Purchase Cards
020 Shipping and Receiving of Coolers, Supplies and Equipment
030 Sample Coordination by the RSCC
050 State Drinking Water Laboratory Certification Program Oversight
SAMPLE CONTROL
110 Sample Receipt, Login, and Storage
112 Sample Receiving in the Absence of the Sample Custodian
115 Chemical Preservation upon Receipt at the Laboratory
125 Aqueous Sample Disposal
130 Glassware Cleaning Procedures
150 Soil and Sediment Homoginization
CHEMISTRY: ORGANIC SAMPLE AND STANDARDS PREPARATION
205 Preparation of Organic-free Method Blank Water
239 Recycling Used Methylene Chloride
260 Gel-Permeation Chromatography (GPC) Clean-up
275 Extraction of Water Samples using CLLE
285 Soxhlet Extraction of Solid Samples
290 Extraction of Soil Samples Using PFE (Pressurized Fluid Extraction - ASE)
295 Waste Dilution
CHEMISTRY: ORGANIC SAMPLE ANALYSIS
305 Volatile Organics Analysis
311 Analysis of Volatile Organic Compounds in Air and Soil Vapor
312 SUMMA Canister Leak Check and Cleaning
314 TO15 SIM-Analysis of Volatile Organic Compounds in Air
315 Semivolatile Organics Analysis
325 Determination of Dissolved Gases in Water
330 Pesticides & PCB (Water, Sediment, Soil) by GC
335 Polychlorinated Biphenyls (PCBs) by GC
350 Analysis of EDB and DBCP in Water by Micro-extraction and GC
353 Low-level Volatiles
354 Volatile Organic Analysis in Water
375 Low-level Semi-Volatile Organics Analysis
380 Purgeable Aromatics and Hydrocarbons by GC PID/FID
385 TPH/DRO Analysis by GC/FID
CHEMISTRY: INORGANIC SAMPLE AND STANDARDS PREPARATION
250 TCLP Extraction
252 California Waste Extraction Test (WET)
254 SPLP Extraction
256 Bioaccessibility Extraction
401 Nepholometric Turbidity Measurement
403 Aqueous Sample Preparation for ICP-AES and ICP-MS
405 Preparation of Solid Samples for Metals Analysis
407 Preparation of Leachate
409 Digestion for Lead on Filters
420 Microwave Digestion of Solid Samples
430 Tissue Preparation and Homogenization
CHEMISTRY: INORGANIC SAMPLE ANALYSIS
460 Percent Solids Determination
461 Total Dissolved Solids
462 Total Suspended Solids
463 Settleable Solids
464 Suspended Sediment Concentration
475 Flash Point by Pensky-Martens Closed Tester
503 Analysis of Solids by ICP
505 Determination of Trace Metals in Water by ICP-AES
507 Trace Metals by ICP-MS
509 Determination of Lead on Filters by ICP-MS
515 Mercury Analysis by Cold Vapor Atomic Absorption
517 Analysis of Solid Samples for Mercury
525 Hexavalent Chromium in Water by Ion Chromatography
530 Anions by Ion Chromatography
531 Analysis of Perchlorate by Ion Chromatography
532 Cyanide Analysis
535 Mercury in Solids by Thermal Decomposition, Amalgamation and AAS
537 Analysis of Perchlorate by LC-MS-MS
545 Specific Conductance
553 Determination of TOC in Water by EPA Method 415.3
560 Total Alkalinity by Titrimetric Method
565 Sulfide Analysis by Titration
570 Hardness, Total (mg/l as CaCO3)
575 Determination of Ferrous Iron by Titration with Dichromate
580 pH Determination
582 pH in Solid Samples
590 Ammonia Analysis
592 Total Kjeldahl Nitrogen in Water
594 Analysis of Nitrite and Nitrate in Water by Cadmium Reduction
596 Analysis of Total Phosphorus in Water
SAFETY, HEALTH & ENVIRONMENTAL COMPLIANCE: STANDARD PRACTICES
704 Inspection Procedures for Emergency Eyewash and Shower Units
706 Laboratory Waste Management
712 Inspection Procedures for the Satellite and 90-Day Hazardous Waste Accumulation Areas
716 Cleaning and Recycling of Empty Containers
730 Neutralization of Aqueous Wastes
745 Maintenance and Inspection of Spill Cleanup Equipment
747 Laboratory Chemical Inventory Maintenance
748 Chemical Adoption Program
750 Inspection Procedures for Fire Extinguishers
760 Maintenance and Upkeep of Radioactive Sources
765 Procedure for Spill Response and Exposure Control for Methylene Chloride
770 Handling Cryogenic Materials
775 Handling and Disposal of Broken Glassware
780 Procedure for Chemical Spill Response and Cleanup
785 Procedure for Storage, Monitoring and Handling of Peroxide Forming Chemicals
QUALITY ASSURANCE
805 Refrigerator temperature monitoring
810 Internal Audits of Laboratories
817 Accuracy Check of Laboratory Thermometers
820 Lab Discrepancy/Corrective Action Report Procedure
825 Deionized water monitoring
830 Notification of exceeding safe drinking water mcl
835 Chromatographic integration procedures
840 Notebook documentation and control
845 Final Chemistry Data Review & Report Preparation
850 Preparation and usage of SOPs
880 Initial & continuing demonstrations of proficiency (Chemistry)
885 Initial & continuing demonstrations of proficiency (Biology)
890 Calibration & maintenance of automatic pipets
892 Initial Instrument Calibration Guidelines
BIOLOGY: MARINE TECHNICAL
1001 Sea urchin & sand dollar fertilization toxicity test
1004 Red abalone larval development toxicity test
BIOLOGY: FRESHWATER TECHNICAL - CHRONIC
1020 Fathead minnow, larval survival & growth test
1022 Green alga, growth test
BIOLOGY: FRESHWATER TECHNICAL - ACUTE
1030 Fathead minnow acute survival test
1032 Daphnia magna acute survival test
BIOLOGY: GENERAL ACTIVITY
1060 Calibration and Operation of pH meter
1061 Calibration and Operation of Dissolved Oxygen Meters
1063 Calibration and Operation of Conductivity Meters
1067 Calibration and Operation of Refractometers
1073 Calibration & Operation of Oakton Portable pH/CON 10 Series Meter
1080 Preparation of standard dilution water (freshwater)
1081 Preparation of foods (freshwater)
1082 Culturing of selenastrum capricornatum
1085 Glass & Plasticware Cleaning for Marine Toxicity Testing
1086 Kelp collection, preparation & feeding
BIOLOGY: MICROBIOLOGY
1101 Membrane filtration coliform analysis
1102 Multiple-tube fermentation analysis for total and fecal coliform
1103 Colilert coliform and E. coli water analysis
1106 Volunteer monitoring of surface waters for bacteria
1107 Enterolert entercocci water analysis
1108 Heterotrophic plate count for bacteria in water
1109 Microbiology Autoclaves
BIOLOGY: MISCELLANEOUS BIOLOGY
1131 Analysis of Ash Free Dry Mass
1133 Biochemical oxygen demand-BOD
1134 Chlorophyll
1135 Total & free residual chlorine - Hach DPD
FIELD SAMPLING: WATER
1220 Groundwater Monitoring Well Sampling
1222 Routine Sampling and Analysis of Drinking water
MISCELLANEOUS: IMMUNOASSAY
1305 Microcystin by ELISA Analysis

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